Introduction

Fluorescence is not only used for lighting, but is also widely used in other applications, such as biochemistry and medicine, printing anti-counterfeiting technology, gems and mineralogy, etc. In the excited state dynamics process of a substance, by measuring the photofluorescence lifetime of the substance and combining it with the fluorescence quantum yield, we can obtain some rate constants during the radiative and non-radiative transition processes. These important parameters can help us further understand the substance. Through practical operations in this station, students will understand the characteristics of the transient fluorescence station and the relationship between fluorescent substances and fluorescence lifetime.

Training Content

Learn and master the measurement principles of time-resolved fluorescence and fluorescence lifetime;

Construction of nanosecond time-resolved fluorescence detection system;

Measurement of time-resolved fluorescence of the sample to be tested;

Fluorescence lifetime fitting analysis.